Immunostaining Protocol: P-Stat3 (Xenograft and Mice)

An improved and versatile immunosuppression protocol for the development of tumor xenograft in mice.

BACKGROUND The objective of the present study was to evaluate the efficacy of a simple, versatile and cost-effective immunosuppression protocol, using cyclosporine, ketoconazole and cyclophosphamide drug regimen to develop human tumor xenograft in mice. MATERIALS AND METHODS Cyclosporine, ketoconazole and cyclophosphamide drug regimen was administered to C57BL/6 mice to induce immunosuppressi...

Ovarian Tumor Xenograft in Mice

Elmezzi Graduate School of Molecular Medicine, Manhasset, NY, United States. The Center for 6 Immunology and Inflammation, Feinstein Institute for Medical Research, North Shore-LIJ Health 7 System, Manhasset, NY, United States. Hofstra North Shore-LIJ School of Medicine, Manhasset, NY, 8 United States. Department of Pathology, Massachusetts General Hospital, Boston, MA, United States. 9 Divisio...

Technical report Protocol for ultrarapid immunostaining of frozen sections

Rapid immunostaining of frozen sections within a tolerable time span would be very helpful for intraoperative diagnosis. A protocol was therefore established using the enhanced polymer one-step staining (EPOS) system (Dako) with antibodies against leucocyte common antigen (LCA), cytokeratin (CK), and anti-melanoma (MEL). Best results with reliable and specific immunostaining and a labelling int...

IgA Nephropathy and Significance of Immunostaining Data

Hypothalamus-Olfactory System Crosstalk: Orexin A Immunostaining in Mice

It is well known that olfaction influences food intake, and conversely, that an individual's nutritional status modulates olfactory sensitivity. However, what is still poorly understood is the neuronal correlate of this relationship, as well as the connections between the olfactory bulb and the hypothalamus. The goal of this report is to analyze the relationship between the olfactory bulb and h...